Achieving stable myocardial regeneration after apical resection in neonatal mice |
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| Authors: | Yandong Li Jie Feng Yan Li Shengshou Hu Yu Nie |
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| Institution: | 1. State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Disease, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China;2. Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan, China |
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| Abstract: | The neonatal heart completely regenerates after apical resection (AR), providing a desirable research model to study the mechanism of cardiac regeneration and cardiomyocyte proliferation. However, AR-induced neonatal heart regenerative phenomenon is controversial due to the variation of operative details in different laboratories. Here, we provide an optimized AR operation procedure with stable regeneration and high survival rate by achieving heart exposure, normalizing myocardium cut-offs, and reducing operation duration. We also established a whole-heart-slice approach to estimate the myocardial regeneration after the AR operation, which ensures no false-negative/positive results. The combination of the optimized AR operation and the whole-heart-slice analysis provides a stable system to study neonatal heart regeneration and cardiomyocyte proliferation in situ. |
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| Keywords: | apical resection heart regeneration whole-heart-slice |
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