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Unravelling myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS): Gender-specific changes in the microRNA expression profiling in ME/CFS |
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| Authors: | Amanpreet K. Cheema Leonor Sarria Mina Bekheit Fanny Collado Eloy Almenar-Pérez Eva Martín-Martínez Jose Alegre Jesus Castro-Marrero Mary A. Fletcher Nancy G. Klimas Elisa Oltra Lubov Nathanson |
| Affiliation: | 1. Institute for Neuro Immune Medicine, Dr. Kiran C. Patel College of Osteopathic Medicine, Nova Southeastern University, Fort Lauderdale, FL, USA Department of Nutrition, Dr. Kiran C. Patel College of Osteopathic Medicine, Nova Southeastern University, Fort Lauderdale, FL, USA;2. Institute for Neuro Immune Medicine, Dr. Kiran C. Patel College of Osteopathic Medicine, Nova Southeastern University, Fort Lauderdale, FL, USA;3. Halmos College of Natural Sciences and Oceanography, Nova Southeastern University, Fort Lauderdale, FL, USA;4. Department of Veterans Affairs, Miami VA Healthcare System, Research Service, Miami, FL, USA South Florida Veterans Affairs Foundation for Research and Education Inc, Fort Lauderdale, FL, USA;5. Escuela de Doctorado, Universidad Católica de Valencia San Vicente Mártir, Valencia, Spain;6. National Health Service, Manises Hospital, Valencia, Spain;7. Vall d'Hebron University Hospital, Vall d'Hebron Research Institute, Universitat Autónoma de Barcelona, Barcelona, Spain;8. Institute for Neuro Immune Medicine, Dr. Kiran C. Patel College of Osteopathic Medicine, Nova Southeastern University, Fort Lauderdale, FL, USA Department of Veterans Affairs, Miami VA Healthcare System, Research Service, Miami, FL, USA South Florida Veterans Affairs Foundation for Research and Education Inc, Fort Lauderdale, FL, USA;9. School of Medicine, Universidad Católica de Valencia San Vicente Mártir, Valencia, Spain |
| Abstract: | Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a multisystem illness characterized by medically unexplained debilitating fatigue with suggested altered immunological state. Our study aimed to explore peripheral blood mononuclear cells (PBMCs) for microRNAs (miRNAs) expression in ME/CFS subjects under an exercise challenge. The findings highlight the immune response and inflammation links to differential miRNA expression in ME/CFS. The present study is particularly important in being the first to uncover the differences that exist in miRNA expression patterns in males and females with ME/CFS in response to exercise. This provides new evidence for the understanding of differential miRNA expression patterns and post-exertional malaise in ME/CFS. We also report miRNA expression pattern differences associating with the nutritional status in individuals with ME/CFS, highlighting the effect of subjects' metabolic state on molecular changes to be considered in clinical research within the NINDS/CDC ME/CFS Common Data Elements. The identification of gender-based miRNAs importantly provides new insights into gender-specific ME/CFS susceptibility and demands exploration of sex-suited ME/CFS therapeutics. |
| Keywords: | exercise gender immune function inflammation microRNAs myalgic encephalomyelitis/chronic fatigue syndrome peripheral blood mononuclear cell |