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microRNA-30a attenuates TGF-β1–induced activation of pulmonary fibroblast cell by targeting FAP-α |
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| Authors: | Geting Wu Bin Xie Can Lu Chen Chen Jianhua Zhou Zhenghao Deng |
| Institution: | 1. Department of Pathology, Xiangya Hospital, Central South University, Changsha, China;2. Department of Pathology, Xiangya Hospital, Central South University, Changsha, China
Department of Pathology, School of Basic Medicine, Central South University, Changsha, China |
| Abstract: | Idiopathic interstitial pulmonary fibrosis is a common diffuse interstitial lung disease and has poor prognosis. And one of the pathological features of it is persistent fibroblast activation. It was reported that microRNA-30a was down-regulated in bronchoalveolar lavage fluid from idiopathic pulmonary fibrosis patients. But whether miR-30a is involved in fibroblast activation and its specific mechanism is unclear. In this study, we aimed to investigate the role of miR-30a in fibroblast activation induced by TGF-β1. We found miR-30a could targetedly suppress FAP-α expression. In MRC5 cells, miR-30a was not only involved in regulating the expression of FAP-α, col1a and α-SMA induced by TGF-β1 but also had a role in cell proliferation with or without TGF-β1 treatment via regulating FAP-α expression. Thus, the results indicated that miR-30a alleviated fibroblast activation by regulating the expression of FAP-α. |
| Keywords: | FAP-α miR-30a MRC5 pulmonary fibrosis TGF-β1 |