ELISA for detection of IgM and IgG antibodies to sandfly fever sicilian virus |
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| Institution: | 1. Department of Virology, National Bacteriological Laboratory, S-105 21 Stockholm, Sweden;2. Department of Virology, Karolinska Institute c/o SBL S-105 21 Stockholm, Sweden;3. National Defense Research Establishment, FOA-5, S-172 90 Sundbyberg Sweden;1. Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana 70803, USA;2. Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California 95616, USA;1. Servicio de Microbiología, Hospital Universitari Vall d’Hebron, PROSICS Barcelona, Universitat Autònoma de Barcelona, España;2. Unidad de Medicina Tropical, Hospital Universitari Vall d’Hebron-Drassanes, PROSICS, Barcelona, España;1. Department of Structural Biology, Weizmann Institute of Science, Rehovot 76100, Israel;5. Department of Chemistry and Macromolecular Assembly Institute, College of Staten Island of the City University of New York, Staten Island, NY 10314, USA;6. PhD Programs in Biochemistry and Chemistry, The Graduate Center of the City University of New York, New York, NY 10016, USA;1. Unidad Micología del Hospital de Infecciosas F. J. Muñiz, Buenos Aires, Argentina;2. Programa de Clínica Estomatológica del Hospital de Odontología José Dueñas, Buenos Aires, Argentina;1. Department of Ophthalmology and Visual Sciences, John A. Moran Eye Center, University of Utah, Salt Lake City, Utah;2. Medical University of South Carolina, Charleston, South Carolina |
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| Abstract: | - •An enzyme-linked immunosorbent assay (ELISA) was developed to detect specific human immunoglobulin G and M antibodies to sandfly fever Sicilian (SFS) virus. Acute and early convalescent serum pairs with ⩾ 7 days between the 2 specimens were available from 20 patients and all showed significant optical density (OD) increase and significant titre rise (⩾ 4-fold) by IgG ELISA. However, negative or borderline-positive sera were found as late as 11 days after onset of symptoms when tested by IgG ELISA.
- •Specific IgM antibodies were detected during the first week of symptoms, and maximum OD values were obtained during the first 4 weeks after onset of disease. The IgM OD values declined over the following 3–9 months. All sera collected later than 14 months post-onset were negative by IgM ELISA.
- •The combination of early antibody response and the need to test only one serum specimen gives IgM ELISA an advantage over IgG ELISA in patient diagnosis.
- •The IgG ELISA was also evaluated as a seroepidemiological tool and compared to a plaque reduction neutralization test (PRNT) using sera from a normal Cypriot population. Of 183 sera tested, 34 (19%) were positive in plaque reduction neutralization tests (PRNT) and 113 (62%) by IgG ELISA. A number of PRNT-negative sera were strongly positive by IgG ELISA and also by indirect immunofluorescence test, which may suggest the presence of a virus related to SFS in Cyprus which has not yet been isolated.
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