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Treatment of lipid-containing wastewater using bacteria which assimilate lipids
Institution:1. Cadham Provincial Public Health Laboratory, Winnipeg, MB, Canada;2. Departments of Medical Microbiology & Infectious Diseases, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, Canada;3. Department of Immunology, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, Canada;1. Food Science and Technology, Kasetsart University, Bangkok 10900, Thailand;2. Health Sciences Research Centre, Life Sciences Department, Whitelands College, University of Roehampton, UK;1. Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science & Engineering, Shandong University, Jinan 250100, PR China;2. Department of Environmental Science, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, PR China;3. Research Centre of Lake Environment, Chinese Research Academy of Environmental Sciences, Beijing 100012, PR China
Abstract:Bacteria which grew in a medium containing olive oil as a sole source of carbon were isolated from two meat plants in the Sendai district of Japan. All of the isolates tested assimilated beef tallow, lard, olive oil and used salad oil as a sole carbon source in shaking cultures. One of the isolates, strain 351, digested lipids most efficiently, as shown by the amount of n-hexane extracts that remained. This bacterium was identified as Bacillus sp. A new and efficient laboratory-scale apparatus for the biological treatment of lipid-containing wastewater was devised using strain 351. The apparatus consisted of a water circulation system for the primary treatment of the water, in which strain 351 was inoculated, and an ordinary aeration tank using activated sludge as a secondary treatment. Lipids in the wastewater could be almost completely removed by this apparatus without physical treatment. On the other hand, an ordinary aeration system in the laboratory using an air stone and air pump resulted in the floating of lipids, and was not successful in digesting lipids even in the presence of strain 351.
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