Bacterial assimilation of polytetramethylene glycol |
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| Institution: | 1. Department of Chemistry, University of Kashmir, Srinagar 190006, J&K, India;2. Department of Chemistry, Government Degree College Pulwama, Department of Higher Education, J&K 192301, India;1. Department of Applied Chemistry, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan;2. Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan;3. Research Center for Solar Energy Chemistry, Osaka University, 1-3, Machikaneyama, Toyonaka, Osaka 560-8531, Japan |
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| Abstract: | Two bacterial strains which assimilate polytetramethylene glycol (PTMG) as a carbon and energy source were isolated from soil samples. They were identified as Alcaligenes denitrificans subsp. denitrificans and Xanthomonas maltophilia. They could also assimilate polyethylene glycol 400, but not polypropylene glycol 670. A. denitrificans did not have any additional nutritional requirement for growth, but X. maltophilia required methionine. Optimum growth was observed on 0.2% of PTMG 265 at pH 5.0, showing maximum growth after 5–7 d. The degrees of polymerization of PTMG 200 or 265 were analyzed by high-performance liquid chromatography and identified to be monomer-octamer by mass spectrometry. The growing and resting cells of A. denitrificans or X. maltophilia also degraded PTMG 200 or 265 completely at a concentration of 0.1–0.2%. Cell extracts of A. denitrificans had PTMG dehydrogenase activity linked with an artificial electron acceptor, such as 2,6-dichloroindophenol, etc., and phenazine methosulfate. |
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