Characterization of an avian rotavirus strain by neutralization and molecular hybridization assays |
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Institution: | 1. Host-Microbe Interactomics, Animal Sciences Group, Wageningen University, De Elst 1, Wageningen, The Netherlands;2. Molecular Ecology, Laboratory of Microbiology, Wageningen University, Stippeneng 4, Wageningen, The Netherlands;1. Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech Republic;2. Biology Centre, Czech Academy of Sciences, Ceske Budejovice, Czech Republic;3. Faculty of Mathematics and Physics, Charles University, Prague, Czech Republic;4. Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom |
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Abstract: | Pigeon rotavirus strain PO-13, which was recently shown to be neutralized by a hyperimmune serum to the prototype serotype 7 virus Ch-2, showed a one-way neutralization cross with turkey rotavirus Ty-1. When its genome was compared by RNA-RNA hybridization under stringent conditions with those of avian and mammalian rotaviruses, PO-13 displayed a low to medium level of homology only with turkey rotavirus strains Ty-1 and Ty-3 but not with chicken rotavirus strain Ch-1. Furthermore, no homology was found between the PO-13 probe and genomic RNA from 11 rotavirus strains which originated from 6 different mammalian species and which represented 6 major mammalian serotypes (1–6). |
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