Effect of polyunsaturated fatty acids on the growth of fish cells in culture |
| |
| Institution: | 1. Department of Biology, Norwegian University of Science and Technology (NTNU), 7010 Trondheim, Norway;2. Nutrimar AS, 7266 Kverva, Norway;1. Grupo de Investigación en Acuicultura (GIA), IU-ECOAQUA, Universidad de Las Palmas de Gran Canaria, Telde, Las Palmas, Canary Islands, Spain;2. Aquaculture Program, Institut de Recerca i Tecnologia Agroalimentàries (IRTA) Centre de Sant Carles de la Ràpita (IRTA-SCR), Sant Carles de la Ràpita, Spain;3. Skretting Aquaculture Research Centre, Stavanger, Norway;1. CIIMAR/CIIMAR-LA, Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos, S/N, 4450-208 Matosinhos, Portugal;2. SPAROS Lda., Área Empresarial de Marim, Lote C, 8700-221, Olhão, Portugal;3. ICBAS, Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal;4. Flatlantic, Rua do Aceiro, S/N, 3070-732 Coimbra, Portugal |
| |
| Abstract: | - 1.1. The effects on growth of supplementing the medium with (n-3) and (n-6) polyunsaturated fatty acids (PUFA) were investigated in Atlantic salmon (AS) and turbot (TF) cell lines.
- 2.2. Neither cell line grew in the absence of serum, and addition of increasing percentages of serum resulted in graded increases in cell growth in both cell lines.
- 3.3. The growth of AS cells was stimulated by supplementing the medium with both (n-6)PUFA and (n-3)PUFA at 5–25 μM, especially 18:3(n-3) and 20:5(n-3).
- 4.4. Intermediate concentrations (15–20 μM) of 18:2(n-6) and 18:3(n-3) increased cell growth in TF cells, although only after 8 days in culture.
- 5.5. In contrast, both (n-3) and (n-6)PUFA at 25 μM tended to inhibit the growth of TF cells, and in longer incubations caused cell death.
- 6.6. The inhibition of TF cell growth rate and, in particular, the cell death induced by 25 μM PUFA could be abolished by the addition of vitamin E to the medium.
|
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
