Chiral analysis of methadone in plasma by high-performance liquid chromatography |
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| Affiliation: | 1. Institute of Neurobiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., bl. 23, 1113 Sofia, Bulgaria;2. Translational Schizophrenia Research Center, Nathan S. Kline Institute for Psychiatric Research, 140 Old Orangeburg Road, Orangeburg, NY 10962, USA;3. Division of Experimental Therapeutics, Departments of Psychiatry and Neuroscience, Columbia University, College of Physicians and Surgeons, New York, NY 10032, USA;4. Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., bl. 23, 1113 Sofia, Bulgaria;5. Center for Neurochemistry, Nathan S. Kline Institute for Psychiatric Research, 140 Old Orangeburg Road, Orangeburg, NY 10962, USA;1. Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani-741235, India;2. Department of Biochemistry and Biophysics, University of Kalyani, Kalyani-741235, India;1. School of Chemistry, University College of Science, University of Tehran, Tehran, Iran;2. Department of Animal, Avian and Marine Biotechnology, National Institute of Genetic Engineering and Biotechnology, P.O. Box 14965-161, Tehran, Iran;1. Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt;2. Pharmacognosy and Pharmaceutical Chemistry Department, College of Pharmacy, Taibah University, Al Madinah AlMunawarah 30001, Saudi Arabia;1. Department of Environmental and Symbiotic Science, College of Agriculture, Food and Environment Sciences, Rakuno Gakuen University, Hokkaido, 069-8501, Japan;2. Australian Centre for Research on Separation Science (ACROSS), School of Natural Sciences, University of Tasmania, Hobart, Tasmania 7005, Australia;3. ARC Centre of Excellence for Electromaterials Science (ACES), School of Natural Sciences, University of Tasmania, Hobart, Tasmania, 7005, Australia;4. ARC Training Centre for Portable Analytical Separation Technologies (ASTech), School of Natural Sciences, University of Tasmania, Hobart, Tasmania, 7005, Australia |
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| Abstract: | A method for the chiral high-performance liquid chromatographic analysis of methadone in plasma has been developed. The method employed organic solvent extraction, enantiomeric separation on a Chiral AGP column, and ultraviolet absorption detection at 212 nm. The intra-day variation in the quantification of methadone enantiomers was less than 9% at the 100 ng/ml level, and the values obtained correlated well with those from a gas chromatographic—mass spectrometric method. Results from patients indicate inter- and intra-individual differences in the ratio between l- and d-methadone in plasma during therapy with racemic methadone. In one patient, a higher level of d-methadone in plasma was caused by both faster elimination and lower bioavailability of l-methadone. |
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