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Investigations of phagocytosis concerning the immunological defence mechanism of Mytilus edulis using a sublethal luminescent bacterial assay (Photobacterium phosphoreum)
Affiliation:1. Reims Champagne-Ardenne University, UMR-I02 SEBIO, Campus Moulin de la Housse, 51687 Reims, France;2. Normandie Univ, unilehavre, UMR-I 02 SEBIO, FR CNRS 3730 SCALE, 76600 Le Havre, France;1. Université de Reims Champagne-Ardenne, UMR-INERIS 02 SEBIO (Stress Environnementaux et Biosurveillance des Milieux Aquatiques), Reims, France;2. INRS, Institut Armand Frappier, 531 Boulevard des Prairies, Laval, Québec, H7V 1B7, Canada;3. Institut Universitaire Européen de la Mer, Laboratoire LEMAR, Plouzané, France;4. Centre d''expertise en Analyse Environnementale du Québec, Ministère du Développement Durable, de l’Environnement et de la Lutte Contre les Changements Climatiques, 2700, Rue Einstein, Québec City, Québec, G1P 3W8, Canada;5. Centre d''expertise en Analyse Environnementale du Québec, Ministère du Développement Durable, de l’Environnement et de la Lutte Contre les Changements Climatiques, 850, Boulevard Vanier, Laval, QC, H7C 2M7, Canada;1. Université de Reims Champagne-Ardenne, Normandie Université, ULH, INERIS, SEBIO, UMR-I 02, Reims, France;2. Université Le Havre Normandie, Université de Reims Champagne-Ardenne, INERIS, SEBIO, UMR-I 02, Le Havre, France
Abstract:
  • 1.1. A simple method for the determination of phagocytosis activity using mussel hemocytes by measuring the bioluminescence is presented.
  • 2.2. The immunological defence activity based on phagocytosis is measured and quantified by a luminescent bacterial assay with Photobacterium phosphoreum.
  • 3.3. The measuring system allows us to establish the stress of the immunological defence mechanism of organisms exposed to chemicals and polluted rivers or sewage. Results with reference substances and the phagocytosis indices of exposed mussels from Norwegian aquaculture plants compared to those of mussels from the German Wadden Sea are given as examples.
Keywords:
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