Antiviral activity of carrageenan on hepatitis A virus replication in cell culture |
| |
| Affiliation: | 1. Université J. Fourier, UFR Pharmacie, Service de Bactériologie-Virologie-Immunologie, 38700 La Tronche, France;2. Centre de Recherches du Service de Santé des Armées, Unité de Biologie Moléculaire, BP 87, 38702 La Tronche Cedex, France;1. MOE Key Laboratory of Theoretical Chemistry of Environment, Center for Computational Quantum Chemistry, South China Normal University, Guangzhou 510631, China;2. Institute of Chemical Physics, Beijing Institute of Technology, Beijing 100081, China;3. School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi’an 710139, China;4. Department of Chemistry and Center for Computational Chemistry, University of Georgia, Athens, GA 30602, USA;1. PhD Program in Biological and Health Sciences, Universidad Autónoma Metropolitana (UAM) Iztapalapa-Xochimilco-Cuajimalpa, Mexico City, Mexico;2. Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México (UNAM), Tlalnepantla, Estado de México, Mexico;3. Department of Neurosciences, National Rehabilitation Institute “Luis Guillermo Ibarra Ibarra” (INR) Secretaría de Salud (SSA), Mexico City, Mexico;4. Departament of Reproductive Biology, Universidad Autónoma Metropolitana (UAM) Campus Iztapalapa, Mexico City, Mexico;1. Department of Chemistry, Tsinghua University, Beijing, China;2. State Key Lab of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, China |
| |
| Abstract: | Sulphated polysaccharides such as iota-, lambda- and kappa-carrageenans showed a potent inhibitory effect on the replication of hepatitis A virus (HAV) in the human hepatoma cell line PLC/PRF/5. No cytotoxic effects were detected with concentrations of carrageenans up to 200 μg/ml. The selectivity indices of these substances, calculated as the ratio of the dose that reduced the number of viable cells to 50 % (CD50) to the effective dose that inhibited 50 % of viral antigen expression (ED50), were > 400 with iota-carrageenan, > 222 with lambda-carrageenan and > 10 with kappa-carrageenan. The selectivity index of ribavirin (reference substance) was only 5. The 3 types of carrageenans resulted in concentration-dependent reduction of HAV-antigen expression and HAV infectivity. Iota-and lambda-carrageenan emerged, from the present study, as promising candidates for chemotherapy of acute hepatitis A. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
