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A new method for labelling of monoclonal antibodies and their fragments with technetium-99m
Affiliation:1. Department of Psychiatry and Behavioral Sciences, Medical University of South Carolina, Charleston, SC, United States;2. Division of Child and Adolescent Psychiatry, Massachusetts General Hospital, Boston, MA, United States;3. Cognitive and Clinical Neuroimaging Core, Marijuana Investigations for Neuroscientific Discovery (MIND) Program, McLean Hospital, Boston, MA, United States;4. Department of Psychiatry, Harvard Medical School, Boston, MA, United States;5. Center for Addiction Medicine Co-Director, Massachusetts General Hospital, Boston, MA, United States;2. Sidney Kimmel Comprehensive Cancer Center, Baltimore, MD;1. Laboratory for Radiopharmacy, Ghent University, Ghent, Belgium;2. Dept Diagnostic Sciences, Ghent University, Ghent, Belgium;3. IBiTech-MEDISIP, Dept of Electronics and Information Systems, Ghent University, Ghent, Belgium
Abstract:A new method of labelling of antibodies with technetium-99m is described. The antibody or fragment is derivatized by iminothiolane. Ligand exchange from a labile technetium complex such as TcO(GLU)2 results in >90% of the technetium labelling the antibody. This method appears to be equally applicable to antibodies and fragments.
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