High-performance liquid chromatographic determination of d-amino acid oxidase activity |
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| Affiliation: | 1. School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou 730050, China;2. State Key Laboratory of Applied Organic Chemistry & College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China |
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| Abstract: | A new procedure for the assay of d-amino acid oxidase activity has been developed. α-Ketoisovaleric acid, derived from d-valine, was estimated by high-performance liquid chromatography after reaction with o-phenylenediamine to give the corresponding quinoxalinol derivative. α-Ketovaleric acid was used as an internal standard to ensure the reproducibility of the method. As an example of application, kidney cortex homogenates were analyzed for their d-amino acid oxidase activity. The advantages of the presented procedure for the determination of the enzymatic activity in biological samples compared with previously reported procedures are discussed. |
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