The effect of size of polystyrene particles on their retention within the rat peritoneal compartment,and on their interaction with rat peritoneal macrophages in vitro |
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| Institution: | 1. Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, Liaoning, PR China;2. School of Functional Food and Wine, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, Liaoning, PR China;1. Shandong Provincial Key Laboratory of Animal Resistance Biology, Key Laboratory of Food Nutrition and Safety of Shandong Normal University, College of Life Sciences, Shandong Normal University, Jinan 250014, PR China;2. Department of Food Industry, Shandong Institute of Commerce and Technology, Jinan 250103, PR China;1. Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China;2. Center for Reproductive Medicine, Shengjing Hospital of China Medical University, Shenyang 110022, China;3. Department of Pharmaceutics, College of Pharmacy Sciences, Jilin University, Changchun 130021, China;4. School of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang 110016, China |
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| Abstract: | Polystyrene particles (size range 300nm-3μm diameter) were radioiodinated and their capture by rat peritoneal macrophages measured in vitro. For unmodified particles, most efficient accumulation was observed using a diameter of 600nm (Endocytic Index (E.I.) = 16.4 ± 2.9μl/106cells/h). Particles (3μm diameter) which had been modified to become more hydrophilic by hydroxymethylation showed an increased rate of capture (E.I. = 136.6 ± 91.2μl/106cells/h). Following intraperitoneal administration to rats, unmodified 3μm particles showed selective accumulation in the omentum (18.4% injected dose/g), and this was increased for the hydroxymethylated bead (35.3% dose/g). The smaller (800 nm) particles were better able to leave the peritoneal compartment. Radiolabelled particles isolated from a peritoneal wash after 5h were mostly cell-associated (72–86%, depending on the type of particle). |
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