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Superiority of the use of a pre-incubated complex of primary and second antibody in the radioimmunoassay of thyroid hormones |
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| Institution: | 1. Translational Cancer Research Unit, Oncology Center GZA Hospitals Sint-Augustinus, Oosterveldlaan 24, Antwerp 2610, Belgium;2. Department of Oncology, University of Antwerp, Universiteitsplein 1, Antwerp 2610, Belgium;3. Department of Oncology, Antwerp University Hospital, Wilrijkstraat 10, Edegem 2650, Belgium;4. Department of Medical Oncology, Erasmus MC Cancer Institute, Erasmus University Medical Center and Cancer Genomics Netherlands, Wytemaweg 80, Rotterdam 3015 CN, The Netherlands;5. Department of Oncology, KU Leuven, Herestraat 49, Leuven 3000, Belgium;6. Department of Pathology, Antwerp University Hospital, Wilrijkstraat 10, Edegem 2650, Belgium;2. Department of Anesthesia and Critical Care, The University of Chicago, Chicago, Illinois, USA |
| Abstract: | We report on the superiority of a radioimmunoassay (RIA) system wherein the second antibody (Ab2) is incorporated as a pre-incubated complex with the primary antibody (Ab1) for the assay of haptens like triiodothyronine (T3) and thyroxine (T4). Separation of the antibody bound and free antigen (Ag) was accomplished by 8% polyethylene glycol (PEG) (final concentration) following a single incubation of less than 1 h. The other advantages of this system are a 15-fold reduction in the quantity of Ab2 (without any need for increasing the concentration of PEG) and the consequent savings in cost. |
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