Partial purification and properties of (Na+ + K+)-ATPase from Potamon potamios |
| |
Affiliation: | 1. Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109-0600, USA |
| |
Abstract: | 1. The tissue distribution of the (Na+ + K+)-ATPase in the freshwater/land crab Potamon Potamios was studied.2. Gills were found to display the highest total activity in the whole animal (47%) but the highest specific activity was detected in the heart (15.15 μmol Pi/mg protein/min.).3. All other organs tested were found to have low enzyme activity.4. The freshwater/land crab ATPase enzyme was inhibited by ouabain with a Ki of 0.5 mM.Km values for ATP, Mg2+ and K+ were 1.4, 4.0 and 1.2mM respectively. The enzyme also showed a break in the Arrhenius plot at 23°C.5. A purification method of microsomal ATPase is described involving ultracentrifugation and electrofocusing. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|