Use of digoxigenin-labelled probes for detection and host-range studies of tomato yellow leaf curl geminivirus |
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| Institution: | 1. Instituto di Fitovirologia Applicata, CNR, Strada delle Cacce 73, 10135 Torino, Italy;2. Institut des Sciences Végétales, CNRS, 91198 Gif-sur-Yvette, France;1. Department of Biochemistry, KAHER, Jawaharlal Nehru Medical College, Belagavi, Karnataka, India;2. KLES Dr. Prabhakar Kore Hospital & Medical Research Centre, Belagavi, Karnataka, India;3. Department of Medicine, KAHER, Jawaharlal Nehru Medical College, Belagavi, Karnataka, India;4. Department of Biochemistry, All India Institute of Medical Sciences, Jodhpur, Rajasthan, India;1. Northern Ireland Centre for Stratified Medicine, Biomedical Sciences Research Institute, University of Ulster, C-TRIC Building, Altnagelvin Area Hospital, Glenshane Road, L/Derry, BT47 6SB, Northern Ireland, UK;2. SAAD Centre for Pharmacy and Diabetes, School of Biomedical Sciences, University of Ulster, Coleraine, Northern Ireland, UK;1. Department of Plant Pathology, Division of Agriculture, University of Arkansas System, Fayetteville, AR 72701, United States;2. USDA-ARS, GIFVL, Chatsworth, NJ 08019, United States;3. Agricultural Institute of Slovenia, Hacquetova 17, Ljubljana, Slovenia;4. Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI 48824, United States;5. USDA-ARS, Corvallis, OR 97330, United States;1. College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia, 010019 China;2. Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang, 050035 China;3. Institutes of Genetics and Developmental Biology, Chinese Academy of Science, Beijing, 100101 China;1. Department of Plant Pathology & Microbiology, Texas A&M University, College Station, TX, USA;2. Department of Plant Pathology & Microbiology, Texas A&M AgriLife Research, Weslaco, TX, USA |
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| Abstract: | We studied the host range of tomato yellow leaf curl virus (TYLCV) in some agronomically important tomato species. Transmission tests with the natural vector Bemisia tabaci from tomato to sweet pepper, eggplant, cucumber, melon, zucchini and spinach showed that these species did not develop symptoms and did not support viral replication. These species therefore do not constitutive a reservoir of the virus and can be cultivated as alternative to tomato in the most affected areas.For host-range studies, we used a quick and sensitive dot-blot assay employing non-radioactive DNA probes. This technique, developed for detecting TYLCV in plant extracts, is easily used for diagnosis. The sensitivity of this non-radioactive test was comparable to that of radiolabelled probes. |
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