Purification and characterization of glyoxalase I from Hansenula mrakii |
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| Institution: | 1. Barcelona Institute for Global Health (ISGlobal), Barcelona, Spain;2. Pompeu Fabra University, Barcelona, Spain;3. Spanish Consortium for Research on Epidemiology and Public Health (CIBERESP), Av. Monforte de Lemos, 3-5. Pabellón 11, 28029, Madrid, Spain;4. Population Health Sciences Institute, Newcastle University, Newcastle, UK;5. Section of Epidemiology, Department of Public Health, University of Copenhagen, Copenhagen, Denmark;6. Medical Research Council Integrative Epidemiology Unit at the University of Bristol, Bristol, UK;7. Department of Environmental Sciences, Faculty of Natural Sciences, Vytautas Magnus University, 53361 Akademija, Lithuania;8. Center for Public Health Research, Massey University-Wellington Campus, PO Box 756, Wellington 6140, New Zealand;9. USDA Forest Service, PNW Research Station, 620 SW Main, Suite 502, Portland, OR 97205, USA;10. Population Health Science, Bristol Medical School, University of Bristol, UK;11. Epidemiology and Environmental Health Joint Research Unit, Foundation for the Promotion of Health and Biomedical Research in the Valencian Region, FISABIO-Public Health, FISABIO-Universitat Jaume I-Universitat de València, Av. Catalunya 21, 46020, Valencia, Spain;12. Nursing School, Universitat de València, C/Menendez y Pelayo, s/n, 46010, Valencia, Spain;13. The Generation R Study Group (NA-2915), Erasmus Medical Center, University Medical Center, PO Box 2040, 3000 CA Rotterdam, the Netherlands;14. Department of Obstetrics and Gynaecology, Erasmus MC, University Medical Centre Rotterdam, Rotterdam, the Netherlands;15. Center for Fertility and Health, The Nowegian Institute of Public Health, Oslo, Norway;p. Amsterdam UMC, University of Amsterdam, Department of Public and Occupational Health, Amsterdam Public Health research institute, Meibergdreef 9, Amsterdam, Netherlands;q. Université de Paris Cité, Inserm, INRAE, Centre of Research in Epidemiology and StatisticS (CRESS), F-75004 Paris, France;r. Biodonostia Health Research Institute, Environmental Epidemiology and Child Development Group, 20014, San Sebastian, Spain;s. Ministry of Health of the Basque Government, Sub-Directorate for Public Health and Addictions of Gipuzkoa, 20013, San Sebastian, Spain;t. Faculty of Psychology of the University of the Basque Country, 20018, San Sebastian, Spain;u. Department of Statistics and Operational Research, Universitat de València, Dr. Moliner, 50 46100, Valencia, Spain;v. Department of Pediatrics, Erasmus Medical Center, University Medical Center, PO Box 2040, 3000 CA Rotterdam, the Netherlands;w. Department of Preventive Medicine and Public Health, Faculty of Medicine, University of the Basque Country (UPV/EHU), 48940, Leioa, Spain;x. Université Grenoble Alpes, Institut Albert Bonniot, équipe d’épidémiologie environnementale appliquée à la reproduction et la santé respiratoire, F-38000 Grenoble, France;y. Inserm, Institut Albert Bonniot, équipe d’épidémiologie environnementale appliquée à la reproduction et la santé respiratoire, F-38000 Grenoble, France;z. Bradford Institute for Health Research, Bradford Teaching Hospitals NHS Foundation Trust, Bradford, UK;11. Department of Medical Sciences, University of Turin, CPO-Piemonte, Turin, Italy;12. Department of Genetics and Bioinformatics, Division of Health Data and Digitalisation, Norwegian Institute of Public Health, Oslo, Norway;13. Department of Social Medicine, School of Medicine, University of Crete, Greece;14. EPIUnit - Instituto de Saúde Pública, Universidade do Porto, Rua das Taipas, n° 135, 4050-600 Porto, Portugal;15. Laboratório para a Investigação Integrativa e Translacional em Saúde Populacional (ITR), Universidade do Porto, Rua das Taipas, n° 135, 4050-600, Portugal;1. Zhejiang Provincial Key Lab for Biological and Chemical Processing Technologies of Farm Product, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, 310023, Zhejiang, China;2. Beijing Laboratory of Food Quality and Safety, Beijing Technology and Business University, Beijing, 100048, China;1. Department of Mechanical Engineering, Eindhoven University of Technology, the Netherlands;2. Department of Hydrology and Atmospheric Sciences, University of Arizona, United States;3. Department of Civil and Environmental Engineering, Princeton University, United States;4. Department of Mechanical Engineering, ShangHai JiaoTong University, China;1. Medical College, Guangxi University, Nanning, Guangxi 530004, China;2. Translational Medicine Research Center, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning, Guangxi 530011, China;3. Reproductive Medical Center of Nanning Second People’s Hospital, Nanning, Guangxi 530031, China;4. College of Life Science and Technology, Guangxi University, Nanning, Guangxi 530004, China;5. Department of Basic Medicine, School of Medicine, Xiamen University, Xiamen, Fujian 361102, China;1. Odense University Hospital, Department for Quality, Research and Health Technology Assessment, Sdr. Boulevard 29, 5000 Odense C, Denmark;2. Catholic University of the Sacred Heart, Department of Economics and Management and ALTEMS (Graduate School of Health Economics and Management), Largo F. Vito 1, 00168 Rome, Italy;3. The National Knowledge Center for the Health Services, P.O. Box 7004 St Olavs plass, N-0130 Oslo, Norway;4. Helsinki and Uusimaa Hospital District, P.O. Box 705, 00029 Hus, Finland;5. Ankara Numune Training and Research Hospital, HTA Unit (ANHTA), Ulku Mahallesi Talatpasa Bulvari No. 5, Altindag, 06100 Ankara, Turkey;6. Medical Direction, Tartu University Clinics, L.Puusepp st 1a, Tartu 50406, Estonia;7. Medical Direction, Lausanne University Hospital (CHUV), Bugnon 21, Lausanne VD CH-1011, Switzerland;8. Ludwig Boltzmann Institute für Health Technology Assessment, Garnisongasse 7/20, 1090 Wien/Vienna, Austria;9. Evaluation of Innovation and New Technologies, Hospital Clínic Barcelona, C/ Villarroel 170, 1.7., 08036 Barcelona, Spain |
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| Abstract: | Glyoxalase I was purified from Hansenula mrakii IFO 0895 which was resistant to 25 mM methylglyoxal. The molecular weight of the purified enzyme was calculated to be 38,000 by both gel-filtration of Sephadex G-150 and SDS-PAGE. The enzyme was almost specific to methylglyoxal (Km = 0.91 mM). The activity of the enzyme was not inhibited by metal ion chelators such as EDTA, which is a potent inhibitor for glyoxalase Is from other sources. |
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