Monoclonal antibodies to antigen binding protein of annelids (Lumbricus terrestris) |
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| Institution: | 1. College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China;2. Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource, Nanjing Agricultural University, Nanjing 210095, China;3. National Joint Local Engineering Research Center for Rural Land Resources Use and Consolidation, Nanjing Agricultural University, Nanjing 210095, China;4. Jiangsu Key Laboratory of Low Carbon Agriculture and GHGs Mitigation, College of Resources & Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China |
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| Abstract: | - 1.1. Monoclonal antibodies (mAb) to antigen binding protein (ABP) of the earthworm Lumbricus terrestris have been prepared.
- 2.2. The specificity of mAb for a determinant located outside the antigen binding site was determined and verified in inhibition experiments.
- 3.3. The mAb were used for isolation of a 56 kDa ABP by an immunoprecipitation technique.
- 4.4. The binding of mAb to coelomocytes has demonstrated the existence of two cell populations, one with low and the other with high densities of ABP molecules on the cell membranes.
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