Factors affecting lactic acid production rate in the built-in electrodialysis fermentation,an approach to high speed batch culture |
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| Institution: | 1. Department of Applied Microbial Technology, The Kumamoto Institute of Technology, Ikeda 4-22-1, Kumamoto 860, Japan;2. Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, Hakozaki, Higashi-ku, Fukuoka 812, Japan;1. State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, Jiangsu Province, PR China;2. School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, Jiangsu Province, PR China;3. School of Food Science and Engineering, Qingdao Agricultural University, Qingdao, 266109, Shandong Province, PR China;1. Laboratory of Flavor and Chromatography Analysis, Biological Division, Federal University Sergipe, Sao Cristovao, 49100-000, Brazil;2. Department of Applied Microbiology and Biotechnology, Dr. Harisingh Gour University, Sagar 470003, MP, India;3. Department of Mathematics and Sciences, College of Arts and Applied Sciences, Dhofar University, Salalah, 2112509, Sultanate of Oman;1. Food Process Engineering Group, Wageningen University, P.O. Box 17, 6700 AA, Wageningen, The Netherlands;2. R&D Department, Aviko B.V., P.O. Box 8, 7220 AA, Steenderen, The Netherlands;1. Institute of Food Science and Technology, Chinese Academy of Agriculture Sciences, Beijing 100193, PR China;2. Institute of Food and Nutrition Development, Ministry of Agriculture, Beijing 100081, PR China;3. Academy of Food Industrial Technology of Hebei Jinshahe Flour Group, Beijing 100071, PR China;1. Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, 9 Meiling Road, Hangzhou, 310008, PR China;2. Graduate School of Chinese Academy of Agricultural Sciences, Beijing, PR China;3. School of Biotechnology and Food Science, Anyang Institute of Technology, Anyang, 455000, PR China;4. Key Laboratory of Horticultural Plants Growth, Development and Quality Improvement, Agricultural Ministry of China, Institute of Vegetable Science, Zhejiang University, Hangzhou, 310058, PR China;1. CSIRO Energy Flagship, Private Bag 10, Clayton South VIC 3169, Australia;2. State Key Laboratory of Petroleum Resources & Prospecting, China University of Petroleum (Beijing), Fuxue Road No. 18, Changping, Beijing 102200, China |
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| Abstract: | To avoid cells adhering to the ion-exchange membrane and damage of the cells in electrodialysis fermentation (ED-F), ED-F with a microfiltration (MF) module, was used in lactate fermentation. In this system, after 38 h of cultivation, the dry cell weight (2.89 g/l) and the number of viable cells (4.8 × 109/ml) were about 1.8 and 1.6 times more than those of the control fermentation, respectively. Lactate dehydrogenase activity in this system was maintained at a high level, resulting in reduction of fermentation time to complete batch culture within 38 h. From these results, ED-F with an MF module enabled high speed batch culture. |
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