Imaging of human ovarian tumour xenografts in nude mice using a novel 111In-labelled monoclonal antibody (10B) |
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| Institution: | 1. Department of General Surgery, The First Hospital of Lanzhou University, Lanzhou 730000, PR China;2. Key Laboratory Biotherapy and Regenerative Medicine of Gansu Province, Lanzhou 730000, PR China;3. Medical Frontier Innovation Research Center, The First Hospital of Lanzhou University, Lanzhou 730000, PR China;4. Hepatopancreatobiliary Surgery Institute of Gansu Province, Lanzhou 730000, PR China;5. The First School of Clinical Medicine, Lanzhou University, Lanzhou 730000, PR China;1. Institute for High Pressure Physics, Russian Academy of Sciences, 142190 Troitsk, Russia;2. A.M. Prokhorov General Physics Institute, Russian Academy of Sciences, 38 Vavilov str., 119991 Moscow, Russia;3. National Research Nuclear University MEPhI, Kashirskoye shosse 31, 115409 Moscow, Russia;4. V.A. Kotelnikov Institute of Radio Eng. & Electronics of Russian Academy of Sciences, Vvedenskogo sq. 1, 141190 Fryazino, Russia |
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| Abstract: | Monoclonal antibody (mAb) 10B, directed against the human ovarian adenocarcinoma cell line, HEY, was conjugated with cyclic DTPA anhydride and labelled with 111In. The biodistribution of 111In-DTPA-10B was determined in non-tumour bearing mice and mice bearing subcutaneous (s.c.) and intraperitoneal (i.p.) HEY tumours. The radiolabel was preferentially targeted to s.c. and i.p. tumours in comparison with a control mAb, 111In-DTPA-2G3, which does not bind to HEY cells. Among normal organs, the predominant uptake of radiolabel was into liver and kidney. Subcutaneous tumours were successfully imaged using external gamma scintigraphy following i.v. injection of 111In-DTPA-10B. The results suggest that 111In-DTPA-10B may be a useful agent for the diagnostic imaging of tumour masses in patients with ovarian cancer. |
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