Measurement of plasma catecholamines by high-performance liquid chromatography with electrochemical detection in intensive care patients after dobutamine infusion |
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| Institution: | 1. School of Biotechnology, Jawaharlal Nehru University, New Delhi, India;2. Department of Biotechnology, TERI School of Advance Studies, New Delhi, 110070, India;3. Department of Chemistry, Sri Aurobindo College, University of Delhi, New Delhi, India;1. Department of Food Science and Technology, Pondicherry University, Puducherry, 605014, India;2. Department of Biochemistry, National Institute of Nutrition, Hyderabad, Telangana, 500007, India;3. Department of Agricultural and Environmental Sciences, Tennessee State University, Tennessee, United States;1. Department of Clinical Laboratory, Traditional Medicine Hospital Affiliated to Southwest Medical University, Luzhou, Sichuan 646000, China;2. Department of Cardiovascular Disease, Traditional Medicine Hospital Affiliated to Southwest Medical University, Luzhou, Sichuan 646000, China;3. Department of Microbiology, College of Basic Medical Sciences, Army Medical University (Third Military Medical University), Chongqing 400038, China;1. Institute of Advance Research Studies in Chemical Sciences, University of Sindh Jamshoro, 76080, Pakistan;2. H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan |
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| Abstract: | A procedure for the determination of plasma catecholamine concentrations in critical care patients after dobutamine infusion is presented. A modified chromatographic system is required with an additional washing procedure to achieve maximum sensitivity and stable chromatographic conditions. The influence of storage time on the catecholamine concentrations of plasma samples is reported in detail. A time-dependent decrease in catecholamine concentrations of up to 12 and 39% was found within two and ten months, respectively. |
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