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Non-porous magnetic supports for cell immobilization
Institution:1. Higher Institute of Chemical Technology, Department of Chemical Engineering, Bul. Kl. Ohridski 8, Sofia, Bulgaria;2. Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Bl. 26, Sofia 1113, Bulgaria;1. Faculty of Pharmaceutical Sciences, Sojo University, Kumamoto, Japan;2. Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan;1. College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 200-701, South Korea;2. Bio-Imaging Center, Korea Basic Science Institute, Chuncheon 200-701, South Korea;1. College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan, 030024, PR China;2. College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan, 030024, PR China;1. Department of Metallurgical Engineering and Materials Science, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India;2. Centre for Research in Nanotechnology and Science (CRNTS), Indian Institute of Technology Bombay, Powai, Mumbai 400076, India;3. Department of MRI, Dr. Balabhai Nanavati Hospital and Research Center, Mumbai 400056, India;1. Bioinorganic Chemistry and Rare Earth Laboratories, Department of Chemistry, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, University of São Paulo, Av. Bandeirantes, 3900, 14040-901 Ribeirão Preto, SP, Brazil;2. Department of Physics, Federal University of São Carlos, Av. Trabalhador São-Carlense, 400 – Pq. Arnold Schimidt, 13566-590 São Carlos, SP, Brazil
Abstract:A new method for covering magnetic particles with a stable non-porous layer of a material like zeolite or activated carbon was used for the preparation of support materials with good properties for the immobilization of yeast Saccharomyces cerevisiae cells. The immobilized cells can be used in batch and continuous alcoholic fermentation. A productivity of 35.6 g ethanol/l · h was reached. The adsorption isotherms of the immobilized yeast cells were determined. Yeast cell immobilization on non-porous magnetic supports obeyed the Langmuir isotherm equation. Satisfactory results were obtained also from repeated batch fermentations with fixed cells on supports additionally treated with glutaraldehyde or by simple adsorption.
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