Pectate-lyase fixation and pectate disorganization visualized by immunocytochemistry in Saintpaulia ionantha infected by Erwinia chrysanthemi |
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| Institution: | 1. Royal Botanic Garden Edinburgh, 20A Inverleith Row, Edinburgh EH3 5LR, Scotland, UK;2. University of Edinburgh, King''s Buildings, Mayfield Road, Edinburgh EH9 3JR, Scotland, UK;3. Tokyo Gakugei University, 4-1-1 Nukuikitamachi, Koganei, Tokyo 184-8501, Japan;4. Biomathematics and Statistics Scotland, Invergowrie, Dundee DD2 5DA, Scotland, UK;5. Ryukoku University, Yokotani 1-5, Seta Ohe-cho, Otsu-shi, Shiga 520-2194, Japan |
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| Abstract: | The degradation of leaf tissue of Saintpaulia ionantha by the pathogen Erwinia chrysanthemi strain 3937 was studied during the first 8h after infection. Monoclonal antibodies (JDF 28B1), specific for the isoenzymes of pectate-lyases (PL) of neutral isoelectric point (PLb and PLc), were used for histo- and cytochemical immunolocalization of the secreted PL. The degree of lysis of pectates was checked by using JIM5, a monoclonal antibody that reacts with homogalacturonan sequences.The heterogeneous distribution of the PL labelling among the different cells and along a given wall could indicate the possible segregation of specific polymers susceptible of being recognized by certain isoenzymes. |
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