| 6－Dimethylaminopurine（6－DMAP） Spontaneously Induces Interphase Transition Of Metaphase Mouse Oocytes |
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| 引用本文： | 孙青原,高绍荣,陈大元.6－Dimethylaminopurine（6－DMAP） Spontaneously Induces Interphase Transition Of Metaphase Mouse Oocytes[J].基因组蛋白质组与生物信息学报,1995(1). |
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| 作者姓名： | 孙青原 高绍荣 陈大元 |
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| 作者单位： | State Key Laboratory of Reproductive Biology，Institute of Zoology，China Academy of Sciences，Beijing，100080，P.R. China |
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| 摘 要： | ６－Ｄｉｍｅｔｈｙｌａｍｉｎｏｐｕｒｉｎｅ（６－ＤＭＡＰ）ＳｐｏｎｔａｎｅｏｕｓｌｙＩｎｄｕｃｅｓＩｎｔｅｒｐｈａｓｅＴｒａｎｓｉｔｉｏｎＯｆＭｅｔａｐｈａｓｅＭｏｕｓｅＯｏｃｙｔｅｓ￥ＳＵＮＱｉｎｇ－ｙｕａｎ（孙青原）；ＧＡＯＳｈａｏ－ｒｏｎｇ（高...
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6-Dimethylaminopurine(6-DMAP) Spontaneously Induces Interphase Transition Of Metaphase Mouse Oocytes |
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| SUN Qing-yuan, GAO Shao-rong,CHEN Da-yuan.6-Dimethylaminopurine(6-DMAP) Spontaneously Induces Interphase Transition Of Metaphase Mouse Oocytes[J].Genomics Proteomics & Bioinformatics,1995(1). |
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| Authors: | SUN Qing-yuan GAO Shao-rong CHEN Da-yuan |
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| Abstract: | Protein kinase inhibitor 6-DMAP was used to explore the effect of protein phosphorylation on germinal vesicle breakdown(GVBD) and chromatin/chromosome behavior of mousc oocytes at different stages .Protein phosphorylation induced by 6-DMAP-sensitive protein kinases was not required for GVBD 2 hrs after culture. 6-DMAP stimulated transition to interphase in oocytes after GVBD and inhibited extrusion of the first polar body(PB1) and the second polar body(PB2). This drug also induced the reformation of nucleus in metaphase II(M II) oocytes spontaneously,without the release of cortical granules(CGs), a result that is different from the conclusion obtained before. |
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| Keywords: | Protein kinase inhibitor Germinal vesicle breakdown Phase transition Cortical granule |
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