| 5-氟尿苷的微生物转化 |
| |
| 引用本文： | 阮期平,周长林,窦洁,吴梧桐.5-氟尿苷的微生物转化[J].微生物学报,2002,42(1). |
| |
| 作者姓名： | 阮期平 周长林 窦洁 吴梧桐 |
| |
| 作者单位： | 中国药科大学生物制药学院,南京,210009 |
| |
| 基金项目： | 国家医药管理局资助项目 |
| |
| 摘 要： | Conditions for biotransformation and purification of FUR were investigated.The result showed that when the cell concentration of E.aerogenes was 10%(w/v),the temperature and pH were 7.8 and 60℃ respectively,59.7% UR was converted to FUR.It also demonstrated that the ideal procedure for the purification of FUR is silica gel column chromatography by two elution systems (S 1∶CHCl 3∶CH 3OH∶H 2O 61∶13∶1,and S 2;CH 3COOCH 2CH 3∶CH 3OH∶CH 3COOH∶H 2O 12∶3∶3∶2)with the purity and yield of 98.0% and 86.0% …
|
| 关 键 词： | 5-氟尿苷 微生物转化 分离纯化 |
Studies on Biotransformation of 5-FUR |
| |
| Abstract: | |
| |
| Keywords: | |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《微生物学报》浏览原始摘要信息 |
| 点击此处可从《微生物学报》下载免费的PDF全文 |
|
