| 嗜热菌Thermus sp.YBJ-1的分离和淀粉酶基因的克隆 |
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| 引用本文： | 熊鹏钧,文建军. 嗜热菌Thermus sp.YBJ-1的分离和淀粉酶基因的克隆[J]. 生物工程学报, 2004, 20(3): 434-436 |
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| 作者姓名： | 熊鹏钧 文建军 |
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| 作者单位： | 国家海洋局第三海洋研究所,海洋生物工程重点实验室,厦门,361005 |
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| 基金项目： | 国家十五“863”项目 (No.2 0 0 1AA62 0 111)，国家大洋协会项目 (No .4 2 4)~~ |
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| 摘 要： | 从西藏热泉水样分离得到一株嗜热菌(YBJ-1)，其16S rDNA(1511bp)序列与栖热菌(Thermus scotoductus ITI-252T)的同源性为98％。通过PCR技术将Thermus sp．YBJ-1的淀粉酶基因(amyT)全长开放阅读框克隆到T载体。分析表明，amyT的ORF全长为1767bp，编码588个氨基酸。推导的氨基酸序列与嗜热脂肪芽孢杆菌的阿尔法环糊精酶(Bacillus stearothermophilus alpha-eyclodextrinase)和栖热菌Thermus sp．IM6501的麦芽糖淀粉酶(Thermus sp．IM6501 mahogenic amylase)分别有99％和96％的同源性，与嗜热脂肪芽孢杆菌的新普鲁兰酶(neopullulanase)的同源性为81％。
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| 关 键 词： | 嗜热菌 麦芽糖淀粉酶 环糊精酶 新普鲁兰酶 |
| 文章编号： | 1000-3061(2004)03-0434-03 |
| 修稿时间： | 2003-09-09 |
Isolation of Thermophilic Bacteria Thermus sp.YBJ-1 and Cloning of Amylase Gene |
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| XIONG Peng-Jun WEN Jian-Jun+. Isolation of Thermophilic Bacteria Thermus sp.YBJ-1 and Cloning of Amylase Gene[J]. Chinese journal of biotechnology, 2004, 20(3): 434-436 |
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| Authors: | XIONG Peng-Jun WEN Jian-Jun+ |
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| Affiliation: | The Key Laboratory of Marine Biotechnology, The Third Institute of Oceanography, SOA, Xiamen 361005, China. |
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| Abstract: | Thermophilic bacteria strain YBJ-1 was isolated from hot spring samples collected from Yangbajing ,Tibet. The 16sr DNA sequence of YBJ-1(1511bp in length) shares 98% identity with that of Thermus.scotoductus strain ITI-252T.The full-length ORF of amylase gene of YBJ-1(amyT) was amplified by PCR technique and cloned into T-vector. The complete sequence of amyT is 1767bp in length,coding for 588 amino acids. The deduced amino acids share 99% similarity with alpha-cyclodextrinse of Bacillus sterothermophilus,96% with maltogenic amylse of Thermus.sp IM6501,and 81% with neopullulanase of Bacillus sterothermophlus. |
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| Keywords: | thermophile maltogenic amylase cyclodextrinase neopullulanase |
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