Molecular evidence of<Emphasis Type="Italic">Bartonella</Emphasis> DNA in ixodid ticks in Czechia |
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Authors: | K Hercík V Hášová J Janeček P Branny |
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Institution: | Laboratory of Cell Signaling, Cell and Molecular Microbiology Division, Institute ofMicrobiology, Academy of Sciences of the Czech Republic, 142 20 Prague, Czechia. |
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Abstract: | We report the development of a novel method for detection of Bartonella DNA in ixodid ticks. The assay is based on a specific amplification of a part of 16S rRNA gene and 16S-23S rRNA intergenic spacer region of Bartonella sp. by nested PCR and Southern blot hybridization with specific DNA probe; the method is highly sensitive and specific. The screening of 327 unfed ticks collected in different urban and suburban areas of Czechia in 2003-2005 revealed the presence of Bartonella DNA in four Ixodes ricinus individuals (1.2%), two males, one female and one nymph. |
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