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| 大鼠肝移植术后腹腔细菌感染模型的建立与评价 | |
| 引用本文： | 丁隆,杨宇,董家鸿.大鼠肝移植术后腹腔细菌感染模型的建立与评价[J].中国实验动物学报,2010,18(1):69-73,I0007. |
| 作者姓名： | 丁隆 杨宇 董家鸿 |
| 作者单位： | 1. 佳木斯大学附属第一医院普外二科,佳木斯,154003 2. 佳木斯大学基础医学院局部解剖教研室,佳木斯,154007 3. 中国人民解放军总医院肝胆外科,北京,100853 |
| 摘 要： | 目的建立大鼠肝移植术后腹腔感染的模型。方法构建DA大鼠到LEW大鼠的肝移植模型,采用腹腔内细菌注射的方法建立感染模型,通过对大鼠肝功、血气、血细胞计数等各项指标的检测对模型进行综合评价。结果肝移植术后5 d注射细菌,大鼠死亡率高,不利后续研究;术后3 d注射细菌,并选定5×105cfu/mL为最终注射浓度,感染后大鼠的7d存活率累计可达到37.5%左右,随之感染的加重,大鼠状态逐渐变差,直肠温度不断升高,WBC计数也随之增加,pH下降,大鼠出现代谢性酸中毒,肝功能损害进行性加重,肝实质的损害重于胆道的损伤,大约在感染5 d左右相继死亡,多器官病理分析表明,大鼠死亡原因为肝损害,不并发肺脏、肾脏损害。结论采用的腹腔内大肠埃希菌注射建立肝移植术后腹腔细菌感染的模型是比较成功的,可用于相关领域的研究。 |
| 关 键 词： | 肝移植 细菌感染 腹腔 免疫抑制 大鼠 |
Establishment and Evaluation of a Rat Model of Peritoneal Bacterial Infection after Liver Transplantation |
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| DING Long,YANG Yu,DONG Jia-hong.Establishment and Evaluation of a Rat Model of Peritoneal Bacterial Infection after Liver Transplantation[J].Acta Laboratorium Animalis Scientia Sinica,2010,18(1):69-73,I0007. | |
| Authors: | DING Long YANG Yu DONG Jia-hong |
| Institution: | DING Long~1,YANG Yu~2,DONG Jia-hong~3(1.Department of General Surgery,The First Affiliated Hospital of Jiamusi University,Jiamusi 154000,China,2.Department of Regional Anatomy,College of Basic Medicine of Jiamusi University,Jiamusi 154002,3.Department of Hepato-Biliary Surgery,Affiliated Hospital of General Hospital of the Chinese People\'s Liberation Army,Beijing 100853) |
| Abstract: | Objective To establish a rat model of peritoneal bacterial infection after liver transplantation.Methods To construct a dark Agouti rat-to-Lewis(DA-to-LEW) rat model of liver transplantation.Peritoneal bacterial infection in the rats was induced by intraperitoneal injection of bacterial suspension.The liver function,blood gas,blood cell count and other indicators of the rat models were detected.Results There was a high mortality rate in rats with bacterial injection at day 5 after liver transplantation,ther... |
| Keywords: | Liver transplantation Immunological sensitization Bacterial infection peritoneal Rat |
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