|
|||||
|
|
Partial characterization of Ia antigens from murine lymphoid cells |
|
| Authors: | Ellen S Vitetta Jan Klein Jonathan W Uhr |
| Institution: | 1. Department of Microbiology, University of Texas, Southwestern Medical School, 75235, Dallas, Texas 2. Irvington House Institute and Department of Microbiology, N.Y.U. School of Medicine, 10016, New York, N.Y. 3. Departments of Human Genetics and Oral Biology, Schools of Dentistry and Medicine, University of Michigan, 48104, Ann Arbor, Michigan |
| Abstract: | Congenic anti-Ia antisera were used to bind radiolabelled Ia antigens from cells of various strains of mice of knownH-2 haplotype. The results indicate that Ia antigens are proteins of molecular weight 30,000 to 35,000 daltons. The Ia antigens are distinct from known H-2 antigens as judged by independent immunoprecipitation as well as by molecular weight. Ia antigens are synthesized by, and are present on the surface of lymphoid cells as evidenced by incorporation studies using3H-leucine and enzymatic radioiodination of cells, respectively. Tissue distribution of cell surface Ia suggests that Ia antigens are on B cells. Ia antigens were detected in the incubation media of3H-leucine labeled splenocytes suggesting that antigens may be secreted. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |