一次密度梯度超速离心分离人血清四种主要脂蛋白的某些理化性质的研究

 本文对一次密度梯度超速离心获得的四种脂蛋白(VLDL、LDL、HDL_2、HDL_3)进行了理化性质的研究。超速离心分析LDL、HDL_2,HDL_3均呈现一个单一尖锐的上浮峰,上浮速率分别为S_1 6.9和F~0_(1.21) 5.7及2.6。等电点聚焦电泳显示,VLDL主要含载脂蛋白C族,E族和少量A-I,B。HDL_2、HDL_3二者载脂蛋白的种类很相似,但量上略有差异,均以载脂蛋白A-Ⅰ,A-Ⅱ为主,Apoc’s,E次之。VLDL、LDL、HDL_2和HDL_3的化学组分分析与文献报道相似。 作者用本法初步分析了不同性别的各脂蛋白分布图,获得有意义的结果。

SOME PHYSICAL AND CHEMICAL PROPERTIES OF FOUR MAJOR SERUM LIPOPROTEINS ISOLATED BY A SINGLE STEP ULTRACENTRIFUGATION

Some of the physical and chemical properties of four major serum lipoproteins isolated by a single step ultracentrifugation were studied. In analytical ultracentrifugation single peaks of LDL, HDL2 and HDL3 with flotation coefficients of Sf 6.9, F1.210 5.7 and F1.210 2.6 respectively were obtained. Characterization with polyacrylamide gel isoelectric focusing electrophresis (PACIF) showed that the VLDL contained C and E as the major proteins and some apo A and apo B in small amounts.Both HDL2 and HDL3 subfractions were found to contain apo A-Ⅰ and apo A-Ⅱ as the major proteins and apo C'S and apo E as the minor components, besides small amounts of several other apoproteins. PAGIF also revealed that HDL2 contained greater amounts of apo C'S than that were present in HDL3.The chemical compositions of VLDL, LDL, HDL2 and HDL3 agreed well with those repoited in the literature.We have used this method to study the sex difference in pattern of serum lipoproteins with satisfactory results.