Role of the cell wall in the ability of tobacco protoplasts to form callus |
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Authors: | L Schilde-Rentschler |
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Institution: | (1) Department of Biochemistry, University of Cambridge, Tennis Court Road, CB2 1QW Cambridge, U.K.;(2) Fachbereich Biologie der Universität, Postfach 397, D-8400 Regensburg 2, Federal Republic of Germany;(3) Fachbereich Biologie der Universität, Postfach 3049, D-6750 Kaiserslautern, Federal Republic of Germany |
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Abstract: | Cellular membranes from dark grown hypocotyls of Phaseolus aureus Roxb. were separated by centrifugation on a continuous sucrose gradient. Each gradient fraction was monitored for activity of inosine diphosphatase (EC 3.6.1.6) and the ability to transfer glucose from UDP-14C]glucose to endogenous lipids in vitro. The highest incorporation of radioactivity into lipids occurred in a particulate fraction correlated with the Golgi apparatus, sedimenting at sucrose densities of 31.5–33% w/w. Three endogenous lipids were glucosylated in vitro. The two main lipids were characterized as steryl glucoside and acylated steryl glucoside; data from chromatography and hydrolysis of the third lipid suggests that it is dolichyl-monophosphate-glucoside. Steryl glucoside was found to be the main glucoside synthesized, but the proportion of the acylated form increased with time. The results are discussed in the context of the role of the Golgi apparatus as a centre of membrane modification within the plant cell.Abbreviations DMP-mannose
dolichyl monophosphate mannose
- ER
endoplasmic reticulum
- GA
Golgi apparatus
- ID-Pase
inosine diphosphatase |
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Keywords: | Acylsteryl-glucosides Dolichylmonophosphateglucoside Golgi membranes Phaseolus aureus Sterylglucosides |
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