Chemical characteristic of an anticoagulant-active sulfated polysaccharide from Enteromorpha clathrata |
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Authors: | Xiaohui Qi Wenjun Mao Yan Gao Yin Chen Yanli Chen Chunqi Zhao Na Li Chunyan Wang Mengxia Yan Cong Lin Jimiao Shan |
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Affiliation: | Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, 5 Yushan Road, Qingdao 266003, People's Republic of China. |
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Abstract: | A sulfated polysaccharide FEP from marine green alga Enteromorpha clathrata was extracted with hot water and further purified by ion-exchange and size-exclusion chromatography. Results of chemical and spectroscopic analyses showed that FEP was a high arabinose-containing sulfated polysaccharide with sulfate ester of 31.0%, and its average molecular weight was about 511kDa. The backbone of FEP was mainly composed of (1→4)-linked β-l-arabinopyranose residues with partially sulfate groups at the C-3 position. In vitro anticoagulant assay indicated that FEP effectively prolonged the activated partial thromboplastin time and thrombin time. The investigation demonstrated that FEP was a novel sulfated polysaccharide with different chemical characteristics from other sulfated polysaccharides from marine algae, and could be a potential source of anticoagulant. |
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