BA对大麦花药培养中药壁的衰退和植株再生频率的影响

用含20ppm 6-BA的0.1％吐温-80溶液喷施花粉为单核前期的大麦上部叶片和穗部,明显影响大麦花药培养效率。实验结果表明:1)BA处理可明显延缓培养花药的药壁衰退进程。2)BA处理后的花药,在培养期间,其死亡的花粉数比对照大大减少,相反其双核或多核的花粉数比对照明显增加。3)BA处理虽然没有促进大麦花粉愈伤组织的诱导率,但显著地促进愈伤组织的生长。提高愈伤组织成长率,增加可转入分化培养的愈伤组织块数。4)BA处理促进愈伤组织的再分化,尤其是绿苗的分化。

EFFECT OF BENZYLADENINE ( BA ) ON THE DEGENERATION OF THE ANTHER WALL TISSUE AND THE FREQUENCY OF SHOOT REGENERATION FROM CULTURED ANTHERS OF HORDEUM VULGARE L.

The upper leaves of anther donor plants of barley bearing anthers at the early uninucleate stage of pollen were sprayed with 20ppm BA containing 0.1% Tween-80. The effects of BA-spraying on anther cultures were as follows: 1) treated with BA, the degeneration of wall tissue of cultured anthers was delayed. 2) The number of dead pollen grains appeared to decrease and that of binucleate and multinucleate pollen grains increased. 3) Although the percentage of callusing anthers was not influenced by the BA-treatment, 4) the growth of calli and percentage of green shoot differentiation from pollen calli were evidently enhanced.