褐飞虱取食诱导的MAPK3基因在水稻叶鞘组织中的定位

利用Northern杂交技术，对促分裂原活化蛋白激酶基因（MAPK3，BPHiw103）进行了表达分析，同时，针对抗虫水稻B5植株接种褐飞虱若虫48h后的叶鞘组织切片进行了原位定位。Northern杂交结果表明，在褐飞虱取食后，MAPK3 mRNA整体表现为上调的特性。原位杂交显示，褐飞虱取食前，MAPK3在水稻叶的薄壁组织中大量表达；而取食后，在韧皮部表达明显增加，在薄壁组织表达则呈下降趋势。这一点在叶心组织切片中表现最为明显。这些结果说明，水稻在受褐飞虱若虫取食诱导和刺激后，MAPK3的表达在受伤部位急剧增加，推测MAPK3基因可能在水稻对褐飞虱的抗性反应中发挥作用。

In situ Localization of the MAPK3 Gene Induced by Brown Planthopper in Rice Sheath

Northern blot was used to analyze the expression of MAPK3 gene (BPHiw103), and in situ hybridization technique was developed for localization of MAPK3 mRNA in rice sheath fed by brown planthopper (BPH) after48 h. The results revealed that the expression of MAPK mRNA was on the whole up-regulated after BPH feeding. In situ hybridization indicated that it was mostly expressed in parenchyma before BPH feeding, while fed by BPH, this gene was mainly increased in phloem, but down-regulated in parenchyma. Such trend was obviouslydemonstrated in the tissue of inner leaves. All the results showed that the MAPK was induced by BPH feeding, and the gene accumulated in injured rice tissue suggested that it might play the role in the resistant response of rice to BPH feeding.