Callus Induction and Plant Regeneration in Alstroemeria |
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Authors: | GONZALEZ-BENITO, M. ELENA ALDERSON, P. G. |
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Abstract: | Callus was induced from mature embryos of Alstroemeria cv. Butterflycultured on MS medium supplemented with 2·0 or 4·0mg dm3 2,4D or picloram and incubated at 25°Cin the dark. The effect of auxin concentration and precultureof embryos was studied. Callus was capable of regeneration aftertransfer to MS medium containing 4.0 mg dm°3 BAP. Shootsand whole plantlets were regenerated. The effect of growth regulators,used in the callus induction medium and the regeneration medium,on plant regeneration was studied Key words: Alstroemeria, callus, plant regeneration. |
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