Isolation and characterization ofSporomusa acidovorans sp. nov., a methylotrophic homoacetogenic bacterium |
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Authors: | Bernard Ollivier Ralf Cordruwisch Anna Lombardo Jean-Louis Garcia |
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Institution: | (1) Institute of Microbiology, M. Curie-Sklodowska University, Akademicka 19, PL-20-033 Lublin, Poland |
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Abstract: | Exopolysaccharides (EPS) of nodulating strains of Rhizobium trifolii and Rhizobium leguminosarum added to red clover seedlings before inoculation reduced the number of nodules. The inhibition of the nodulation was correlated with the amount of EPS. The preparations of EPS from mutants defective in early stages of nodulation (Roa- or Hac-) did not affect the nodulation, whereas EPS from mutants deficient in late stages (post Hac-) exerted an inhibitory effect.Inactive preparation of EPS contained less O-acetyl groups and pyruvic acid residues. Deacetylation and depyruvylation of EPS from R. trifolii Nod+ abolished it inhibitory effect. It was concluded that noncarbohydrate substitutions (acetate, pyruvate) are involved in EPS effect.Abbreviations CPS
capsular polysaccharide
- EPS
exopolysaccharide
- LPS
lipopolysaccharide
- Nod
nodulation
- Fix
nitrogen fixation
- Hac
root hairs curling
- Roa
root adhesion |
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Keywords: | Exopolysaccharides Nodulation Rhizobium trifolii O-acetyl groups Pyruvic acid |
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