Efficient targeting of adenoviral vectors to integrin positive vascular cells utilizing a CAR-cyclic RGD linker protein |
| |
Authors: | Krom Y D Gras J C E Frants R R Havekes L M van Berkel T J Biessen E A L van Dijk K Willems |
| |
Institution: | Department of Human Genetics, Leiden University Medical Center, Leiden, The Netherlands. Y.D.Krom@lumc.nl |
| |
Abstract: | Vascular smooth muscle (VSMC) and endothelial cells (EC) are particularly resistant to infection by type 5 adenovirus (Ad) vectors. To overcome this limitation and target Ad vectors to ubiquitously expressed alpha(V)beta(3/5) integrins, we have generated a linker protein consisting of the extracellular domain of the coxsackie adenovirus receptor (CAR) connected via avidin to a biotinylated cyclic (c) RGD peptide. After optimization of CAR to cRGD and to Ad coupling, infection of mouse heart endothelial cells (H5V) could be augmented significantly, as demonstrated by 600-fold increased transgene expression levels. In EOMAs, a hemangioendothelioma-derived cell line, the fraction of infected cells was enhanced 4- to 6-fold. Furthermore, the fraction of infected primary mouse VSMC was increased from virtually 0% to 25%. Finally, in human umbilical vein endothelial cells, the number of GFP positive cells was enhanced from 2% to 75%. In conclusion, CAR-cRGD is a versatile and highly efficient construct to target Ad vectors to both transformed and primary VSMC and EC. |
| |
Keywords: | Adenovirus Targeting Cyclic RGD peptide Endothelial cells Vascular smooth muscle cells |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|