Polyamine Sparing May Be Involved in the Prolongation of Cell Division Due to Inhibition of Phenylpropanoid Synthesis in Cytokinin-Starved Soybean Cells |
| |
Authors: | J C Mader D E Hanke |
| |
Institution: | (1) Department of Plant Sciences, University of Cambridge, Downing St. CB2 3EA, Cambridge, UK, GB |
| |
Abstract: | Effects on growth, mostly of an inhibitory nature, have been attributed to phenolic compounds in vivo and in vitro. This
suggests that l-α-aminooxy-β-phenylpropionic acid (l-AOPP), a competitive inhibitor of phenylalanine ammonia-lyase (PAL), the enzyme controlling the first step in phenylpropanoid
synthesis, might stimulate growth in soybean suspension cultures (Glycine max, cv. Acme). The promotive effect of l-AOPP, measured as an increase in cell number, was more clearly detected in the growth-limiting condition of cytokinin starvation.
At least one more cell division cycle was completed in the presence of l-AOPP before growth by division ceased and growth continued by expansion only. Phenolic acids are known to conjugate with
polyamines, modulating the free levels of these plant growth substances. Thus, the effect of l-AOPP on the titers of free and conjugated polyamines (putrescine, spermidine, and spermine) was investigated by high performance
liquid chromatography in the course of cytokinin starvation. An increased level of free putrescine was detected in the presence
of l-AOPP relative to controls, especially in the initial period before growth became restricted to cell expansion. The decrease
in free putrescine associated with the cessation of cell division was temporarily delayed, suggesting that an interaction
between phenolic acids and polyamines is involved in the mechanism of growth promotion by l-AOPP.
Received July 30, 1996; accepted January 28, 1997 |
| |
Keywords: | ,Glycine max—,Polyamines—,Phenolic compounds—,l-AOPP—,Cell division—,Cell elongation—,Cytokinin starvation |
本文献已被 SpringerLink 等数据库收录! |
|