Isoform-Specific Membrane Translocation of Protein Kinase C After Ischemic Preconditioning |
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Authors: | Kurkinen Kaisa Busto Raul Goldsteins Gundars Koistinaho Jari Pérez-Pinzón Miguel A |
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Institution: | (1) Department of Neurology, University of Miami School of Medicine, Miami, FL, 33101;(2) University of Tampere, Kuopio, Finland |
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Abstract: | Mild cerebral anoxic/ischemic/stress insults promote tolerance and thereby protect the brain from subsequent lethal anoxic/ischemic insults. We examined whether specific activation of PKC , , , or isoforms is associated with ischemic preconditioning (IPC) in rat brain. IPC was produced by a 2-minute global cerebral ischemia. Membrane and cytosolic fractions of the hippocampi were immunoblotted using specific antibodies for PKC, , , and . PKC showed a significant translocation to the membrane fraction from 30 min to 4 h and PKC at 4 h following IPC. In contrast, the membrane/cytosol ratio of PKC showed a tendency to decrease at 30 min and 8 h, and the membrane/cytosol ratio of PKC was significantly decreased from 30 min to 24 h following IPC. These findings indicate PKC isoform-specific membrane translocations in the hippocampus after brief global brain ischemia and suggest that activation of PKC and PKC may be associated with IPC-induced tolerance in the rat hippocampus. |
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Keywords: | Ischemia adenosine anoxia tolerance |
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