Characterization of alpha-melanocyte-stimulating hormone in rat pancreas |
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Authors: | M D Hirsch T L O'Donohue |
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Institution: | Experimental Therapeutics Branch National Institute of Neurological and Communicative Disorders and Stroke National Institutes of Health, Bethesda, MD 20205 USA |
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Abstract: | Reverse-phase high performance liquid chromatography and radioimmunoassay were used to characterize alpha-melanocyte-stimulating hormone (alpha-MSH)-like peptides in rat pancreas. Relative to synthetic alpha-MSH standards, serial dilutions of pancreas extracts showed parallel and concentration dependent displacement of (125I) alpha-MSH from alpha-MSH antibody. Chromatographic separation revealed immunoreactive material coeluting with synthetic N,O-diacetyl alpha-MSH, which accounted for 78% of total alpha-MSH materials in this tissue. The remainder of immunoreactive alpha-MSH coeluted with synthetic alpha-MSH, desacetyl alpha-MSH, or their methionine sulfoxides. In contrast with anterior pituitary, it appears that biosynthetic processing of alpha-MSH from pro-opiomelanocortin (POMC) may be similar in rat pancreas and pituitary intermediate lobe, since their relative alpha-MSH immunoreactive elution profiles were similar. These findings support the hypothesis of tissue specific regulation of biosynthetic processing of POMC. |
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Keywords: | α-MSH Pancreas HPLC RIA Pro-opiomelanocortin (POMC) N-acetylation |
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