Measurement of bacterial gene expression in vivo by laser capture microdissection and quantitative real-time RT-PCR |
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Authors: | Klitgaard Kirstine Jensen Tim K Angen Øystein Boye Mette |
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Affiliation: | National Veterinary Institute, Technical University of Denmark, Bülowsvej 27, DK-1790 Copenhagen V, Denmark. |
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Abstract: | Due to the relative small number of bacterial pathogens present in an infected host, exploration of pathogen gene expression in vivo is challenging. This study reports the development of a protocol for quantifying bacterial gene expression in vivo in Actinobacillus pleuropneumoniae using laser capture microdissection and real-time quantitative RT-PCR. |
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