紫檀芪对小鼠缺血性脑损伤后脑水肿期神经细胞凋亡的影响

摘要 目的：探索紫檀芪（PTE）对小鼠缺血性脑损伤后脑水肿期神经细胞凋亡的影响。方法：将实验小鼠分为3组即假手术组（sham组）、脑缺血再灌注损伤组（IR组）和紫檀芪治疗组（PTE+IR组），其中PTE于造模前连续5天每天腹腔给药（5 mg/kg）1次；然后于造模后3 d进行脑组织TTC染色并计算脑梗死体积比；于造模后2 h、12 h和1、2、4、6、8、10、12及14 d进行小鼠神经行为学评分；使用TUNEL试剂盒于造模后3、7和14 d检测缺血半暗带和海马的凋亡神经细胞。结果：PTE可减轻脑梗死体积、改善神经行为学评分以及抑制缺血半暗带和海马的神经细胞凋亡。结论：PTE在小鼠缺血性脑损伤后脑水肿期具有明确的神经保护作用，其机制与抑制细胞凋亡有关。

The Effect of Pterostilbene on Neuronal Apoptosis during Brain Edema after Ischemia Reperfusion Injury in Mice

ABSTRACT Objective: To explore the effect of pterostilbene on neuronal apoptosis during brain edema after ischemia reperfusion injury in mice. Methods: The experimental mice were randomly divided into three groups: sham group, IR group and PTE+IR group. The dose of PTE was injected intraperitoneally with 5 mg/kg once a day for 5 days totally. The cerebral infarct image was stained by TTC and the cerebral infarct volume was measured by Image J after IR 3 d. The neurological behavioral scores were tested after IR1/12, 1/2, 1, 2, 4, 6, 8, 10, 12 and 14 d. The neuronal apoptotic percentage of cerebral ischemic penumbra and ipsilateral hippocampus were detected by TUNEL detection kit after IR3, 7 and 14 d. Results: PTE lowered cerebral infarct volume, improved neurological behavioral scores and inhibited neuronal apoptosis of cerebral ischemic penumbra and ipsilateral hippocampus. Conclusion: PTE presents a continuous neuroprotective effect against ischemia reperfusion during cerebral edema in mice by suppression of cell apoptosis.