Acid-soluble chromosomal proteins in maize root and callus cells and after rhizogenesis induction in callus tissues |
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Authors: | Stanka Koleva Elena Marinova Stefka Varadinova Elena Tsikova A Atanassov |
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Institution: | 1. M. Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Acad. Gr. Bonchev Street, Block 21, 1113, Sofia, Bulgaria 2. Institute of Genetics, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria
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Abstract: | Callus tissues originating fromZea mays root meristem, induced for rhizogenesis callus, meristematic and differentiated maize root cells for isolation of nuclei and acid-soluble chromosomal proteins were used. Cytological investigations proved that rhizogenesis begins with the formation of meristematic centres, followed by root differentiation about 5–12 days after the treatment with α-naphtalene acetic acid (NAA). When applying electrophoresis in 15% polyacrylamide gel, differences between the electrophoretic profiles of acid-soluble chromosomal proteins, isolated from root cells and from callus tissues, were established. The main differences concern histone H1 and probably H4. There are no differences between electrophoretic patterns of acid-soluble chromosomal proteins of nonorganized callus and callus induced for rhizogenesis. The possible explanation of these results is discussed. |
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