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An automated procedure for the determination of oxytetracycline |
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| Authors: | R. M. Abdel-Azize B. M. Haroun A. A. Ismail A. M. Eslam |
| Affiliation: | 1. El-Nasr Pharmaceutical Company, Abou Zabal, Cairo, Egypt 2. Faculty of Science, Al-Azhar University, Cairo, Egypt 3. Genetics and Cytology Laboratory, National Research Centre, Dokki, Cairo, Egypt |
| Abstract: | An automated analytical system has been used to determine the amount of oxytetracycline in pharmaceutical products and fermentation broth at a level of 0.1mg/mL. The method is based on the formation of a colour complex between the oxytetracycline and ammonium molybdate in acid media at 80°C. The effect of temperature, concentration of HCl and ammonium molybdate upon the stability of the complex was determined. Standard error of difference is 0.43% for pharmaceutical products and the fermentation broth. |
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