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Dexamethasone influences human clock gene expression in bronchial epithelium and peripheral blood mononuclear cells in vitro |
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| Authors: | Burioka Naoto Takata Miyako Okano Yoko Ohdo Shigehiro Fukuoka Yasushi Miyata Masanori Takane Hiroshi Endo Masahiro Suyama Hisashi Shimizu Eiji |
| Affiliation: | a Division of Medical Oncology and Molecular Respirology, Faculty of Medicine, Tottori University, Yonago, Japanb Department of Clinical Pharmacokinetics, Division of Pharmaceutical Sciences, Graduate School, Kyushu University, Higashi-Ku, Fukuoka, Japanc Department of Pharmacology, Faculty of Medicine, Tottori University, Yonago, Japan |
| Abstract: | We determined whether human peripheral blood mononuclear cells (PBMCs) could be used to analyze clock genes by studying their mRNA expressions in human bronchial epithelium (BEAS-2B) and PBMCs following stimulation by the glucocorticoid homologue dexamethasone (DEX) in vitro. PBMCs were obtained at 10:00 h from two diurnally active (∼07:00 to 23:00 h) healthy volunteers and were evaluated for hPer1 mRNA expression following DEX stimulation in vitro using real time-PCR analysis. DEX stimulation of human BEAS-2B cells and PBMCs in vitro led to a remarkable increase of hPer1 mRNA. The glucocorticoid rapidly affected the expression of hPer1 mRNA in PBMCs, suggesting that human PBMCs may be a useful surrogate marker for the investigation of drug effects on clock genes. |
| Keywords: | Human Beings Clock Genes Glucocorticoid Dexamethasone Per1 Peripheral Blood Mononuclear Cells Circadian Rhythm |
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