Fluorescence titration with apoflavodoxin: a sensitive assay for riboflavin 5'-phosphate and flavin adenine dinucleotide in mixtures. |
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| Authors: | J H Wassink S G Mayhew |
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| Institution: | Dept. of Anatomy University of Connecticut Health Center Farmington, Connecticut 06032 USA |
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| Abstract: | A method is described for determining riboflavin 5′-phosphate (FMN) and flavin adenine dinucleotide (FAD) in mixtures by fluorimetric titration with the FMN-specific apoprotein of flavodoxin from Peptostreptococcus elsdenii. Accurate determinations can be carried out in the presence of a variety of compounds that decrease the fluorescence yield of FMN; the method may therefore be especially useful in the analysis of crude protein-free extracts of biological materials. |
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