Quantitative and qualitative adjustment of thymic T cell production by clonal expansion of premigrant thymocytes |
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Authors: | Le Campion Armelle Lucas Bruno Dautigny Nicole Léaument Sandrine Vasseur Florence Pénit Claude |
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Affiliation: | Institut National de la Santé et de la Recherche Médicale Unité 345 and Laboratoire d'Expérimentation Animale et de Transgénèse, Institut Necker, Paris, France. lecampio@necker.fr |
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Abstract: | In normal mice, single-positive thymocytes proliferate before being exported into the peripheral T cell pool. We measured the in vivo proliferation rates of mature thymocytes in several TCR transgenic mice. Different monoclonal TCR transgenic single-positive thymocytes proliferated at different rates in a given MHC context. Conversely, mature thymocytes expressing a given TCR, generated in mice of different MHC haplotypes, also showed different rates of proliferation. In p59(fyn)-deficient mice, the proliferation rate of mature thymocytes was diminished. Thus, premigrant thymocyte expansion is TCR mediated and depends on TCR affinity for self peptide/MHC ligands. In addition, we show that mature thymocyte expansion is clonotypic, increases the daily thymic T cell output, and modifies the TCR repertoire of newly produced T cells. |
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