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The cell wall polysaccharide of Streptococcus gordonii 38: structure and immunochemical comparison with the receptor polysaccharides of Streptococcus oralis 34 and Streptococcus mitis J22 |
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| Authors: | Reddy G Prabhakar; Abeygunawardana C; Bush C Allen; Cisar John O |
| Institution: | 1Department of Chemistry and Biochemistry, University of Maryland Baltimore County Baltimore, MD 21228, USA 2Laboratory of Microbial Ecology, National Institute of Dental Research Bethesda, MD 20892, USA 3Present address: Department of Biological Chemistry, Johns Hopkins School of Medicine Baltimore, MD 21205, USA |
| Abstract: | As part of our ongoing investigations involving lectinmediatedadhesion among oral bacteria, the receptor polysaccharide fromStreptococcus gordonii 38 was isolated and characterized. Carbohydrateanalysis of the hydrolysed S.gordonii 38 polysaccharide by high-performanceanionexchange chromatography with pulsed amperometric detection(HPAEC-PAD) showed galactose (Gal) (2 mol), N-acetylgalactosamine(GalNAc) (1 mol), rhamnose (Rha) (2 mol), glucose (Glc) (1 mol)and galactosamine-6-phosphate (1 mol). Mild acid hydrolysisof the polysaccharide yielded a heptasaccharide repeating unit.The structure of the heptasaccharide repeating unit was determinedby high-resolution NMR spectroscopy which includes various homonuclear(DOF—COSY, TQF-COSY, NOESY and HOHAHA) and heteronuclearexperiments (HMQC), including linkage assignments by 1H-13Clong-range correlation (HMBC). Complete 1H and 13C NMR assignmentsfor the intact polysaccharide yielded the covalent structureof a heptasaccharide repeating unit: |
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