Indole-3-butyric acid in Arabidopsis thaliana III. In vivo biosynthesis |
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Authors: | Jutta Ludwig-Müller Ephraim Epstein |
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Institution: | (1) Botanisches Institut, J. W. Goethe-Universität, Siesmayerstr. 70, 60054 Frankfurt am Main, Germany;(2) Department of Horticulture, The Volcani Center, ARO, Bel Dagan, 50 250, Israel |
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Abstract: | Indole-3-butyric acid (IBA) was identified by HPLC and GC-MS as one of the reaction products after incubation of sterile cultures of Arabidopsis thaliana seedlings with labeled indole-3-acetic acid (IAA). This is the first demonstration of IBA biosynthesis in a dicotyledonous plant. After 1 h of incubation most of the IBA was found in the free form, while after longer periods of incubation most of it was detected in conjugated forms. Formation of IBA conjugates was inhibited by the addition of unlabeled IBA. The biosynthesis of IBA and its conjugates was followed throughout the development of the seedlings and at different pH values. All parts of the plant (isolated roots, leaves, shoots and flowers) were able to convert IAA to IBA to the same extent.IAA was more readily transported than IBA in mature Arabidopsis plants. Feeding of labeled phenylacetic acid (PAA) and -naphthylacetic acid (NAA) to Arabidopsis seedlings resulted in a new small peak which was hydrolyzed by 7N NaOH, but the formation of compounds with longer side chains (analogous to IBA) could not be detected.Abbreviations IAA
indole-3-acetic acid
- IBA
indole-3-butyric acid
- NAA
-naphthylacetic acid
- PAA
phenylacetic acid |
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Keywords: | Arabidopsis thaliana IBA biosynthesis indole-3-acetic acid indole-3-butyric acid -naphthylacetic acid" target="_blank">gif" alt="agr" align="BASELINE" BORDER="0">-naphthylacetic acid phenylacetic acid |
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